Human adenovirus 2 (ATCC® VR-1786)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus C  /  Product Format: frozen

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Classification Adenoviridae, Mastadenovirus, Human adenovirus C
Agent Human adenovirus 2
Strain ATCC-2011-7
Applications
Respiratory research
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder
Comments
This virus was isolated in cell culture at ATCC.
Effect on Host
CPE, refractile cells with typical lattice formation
Recommended Host
A549 (ATCC CCL-185)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted in VGM to provide an optimal MOI (e.g. MOI 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-6 days
Effect on Host
CPE, refractile cells with typical lattice formation
Name of Depositor ATCC
Source Clinical patient positive for Adenovirus, Virginia, July 2011
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation