P3X63Ag8 (ATCC® TIB-9)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: plasmacytoma; myeloma

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Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Karyotype hyperdiploid; modal number = 65
Derivation
This line was derived from the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody
Comments

The line is resistant to 0.1 mM 8-azaguanine and die in HAT medium.

The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions Temperature: 37°C
Atmosphere: 5% CO2
Isotype IgG1; kappa light chain
Name of Depositor G Kohler, C Milstein
References

Koprowski H, et al. Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc. Natl. Acad. Sci. USA 74: 2985-2988, 1977. PubMed: 268647

Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497, 1975. PubMed: 1172191

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kohler G, et al. Fusion of T and B cells. Somatic Cell Genet. 3: 303-312, 1977. PubMed: 305123

Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377

Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851

Lemke H, et al. Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature 271: 249-251, 1978. PubMed: 74805

. . Eur. J. Immunol. 3: 135-140, 1973.

Secher DS, et al. Spontaneous mutation in tissue culture-chemical nature of variant immunoglobulin from mutant clones of MOPC 21. FEBS Lett. 37: 311-316, 1973. PubMed: 4763339

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Koprowski H, et al. Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc. Natl. Acad. Sci. USA 74: 2985-2988, 1977. PubMed: 268647

Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497, 1975. PubMed: 1172191

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kohler G, et al. Fusion of T and B cells. Somatic Cell Genet. 3: 303-312, 1977. PubMed: 305123

Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377

Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851

Lemke H, et al. Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature 271: 249-251, 1978. PubMed: 74805

. . Eur. J. Immunol. 3: 135-140, 1973.

Secher DS, et al. Spontaneous mutation in tissue culture-chemical nature of variant immunoglobulin from mutant clones of MOPC 21. FEBS Lett. 37: 311-316, 1973. PubMed: 4763339