BL-3 (ATCC® CRL-8037)

Organism: Bos taurus, cow  /  Cell Type: B lymphocyte  /  Disease: lymphosarcoma; leukemia

Permits and Restrictions

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Organism Bos taurus, cow
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Disease lymphosarcoma; leukemia
Age 3 months
Gender male
Strain Hereford
Applications
The cells do not produce bovine leukemia virus.
They are of possible use in making vaccines against bovine leukemia virus.
Clinical Data
male
Comments
The cells do not produce bovine leukemia virus.
They are of possible use in making vaccines against bovine leukemia virus.
Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor Regents of the University of California
References

Theilen GH, et al. Bovine leukemia: establishment and morphologic characterization of continuous cell suspension culture, BL-1. J. Natl. Cancer Inst. 40: 737-749, 1968. PubMed: 4171552

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation
References

Theilen GH, et al. Bovine leukemia: establishment and morphologic characterization of continuous cell suspension culture, BL-1. J. Natl. Cancer Inst. 40: 737-749, 1968. PubMed: 4171552

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438