MYA-4702

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Strain Designations	EN25S01 [CBS 12244, BCRC 23240]
Application	Ferments glucose
Biosafety Level	1
Product Format	frozen
Type Strain	yes

Morphology	On YM medium at 25C after 7 days, colonies creamy white to beige, smooth, with entire margin. Cells globose to ellipsoidal, smooth, small cells 3-3.75µm X 1.5-3µm, large cells 4.5-7.5µm X 4.5-6µm. Pseudohyphae present.

Medium	ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 1245: YEPD
Growth Conditions	Temperature: 20°C to 25°C Atmosphere: Typical aerobic

Sequenced Data	>18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence; GenBank number JQ906769: AAGGATCATTAAAGTATTCTTCAGGCAGCCAGCGCTTCCACAGCGCGGCCGCCTAAACCTTACACACTGTGATTAGTTTTTTTATCATTGCTTTGGCTTGGGGTAACCCGGCCGAAGGTTTTAAACACAAAGATTTTATCTTTTTCTTTTAAACATCAGTCAACGAAGTTTTAAACTATAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCAACGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGGTTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCCCTCGGGTTTGGTATTGAGTGATACTCTGTCCAGGGTTAACTTGAAATAGTGAATTGGCAAGAGTGTGCTTCTGCGGCCTCTTGACTGAGATAATGTATTAGGTTCTACCAACTCGTTATAGCGGTCTAGAAAGTCCAGGCGGCATATTGAGCTCGGCCCAACAACCACCATAAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAG >D1D2 region of the 28S ribosomal RNA gene; GenBank number JQ906764: ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCACCCTCGGTGCCCGAGTTGTAATTTGAAGATAGTTTTCTGGCACTGGCCCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTCTGGCGGGGAGTCCAGTGCTTTGTAGATTTCTATCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTATTCGATCAGACTTGGTGTTTTGTGATTATCTTCCCTTCTTGGGTTGTGCACTCGCATTTCACTGGGCCAGCATCGGTTCGGGTGGCAAGATAATGACATTGGAACGTGGCACCTTCTTCGGATGGTGTGTTATAGCCCTTGTTGATGTTGCCTGCCTGGACCGAGGACTGCGGCTTCTGCCTAGGATGCTGGCGTAATGATCCAATACCG >18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AAGGATCATTAAAGTATTCTTCAGGCAGCCAGCGCTTCCACAGCGCGGCCGCCTAAACCTTACACACTGTGATTAGTTTTTTTATCATTGCTTTGGCTTGGGGTAACCCGGCCGAAGGTTTTAAACACAAAGATTTTATCTTTTTCTTTTAAACATCAGTCAACGAAGTTTTAAACTATAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCAACGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGGTTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCCCTCGGGTTTGGTATTGAGTGATACTCTGTCCAGGGTTAACTTGAAATAGTGAATTGGCAAGAGTGTGCTTCTGCGGCCTCTTGACTGAGATAATGTATTAGGTTCTACCAACTCGTTATAGCGGTCTAGAAAGTCCAGGCGGCATATTGAGCTCGGCCCAACAACCACCATAAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAG >D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCACCCTCGGTGCCCGAGTTGTAATTTGAAGATAGTTTTCTGGCACTGGCCCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTCTGGCGGGGAGTCCAGTGCTTTGTAGATTTCTATCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTATTCGATCAGACTTGGTGTTTTGTGATTATCTTCCCTTCTTGGGTTGTGCACTCGCATTTCACTGGGCCAGCATCGGTTCGGGTGGCAAGATAATGACATTGGAACGTGGCACCTTCTTCGGATGGTGTGTTATAGCCCTTGTTGATGTTGCCTGCCTGGACCGAGGACTGCGGCTTCTGCCTAGGATGCTGGCGTAATGATCCAATACCG
Morphology	On YM medium at 25C after 7 days, colonies creamy white to beige, smooth, with entire margin. Cells globose to ellipsoidal, smooth, small cells 3-3.75µm X 1.5-3µm, large cells 4.5-7.5µm X 4.5-6µm. Pseudohyphae present.

Sequenced Data

>18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence; GenBank number JQ906769:

AAGGATCATTAAAGTATTCTTCAGGCAGCCAGCGCTTCCACAGCGCGGCCGCCTAAACCTTACACACTGTGATTAGTTTTTTTATCATTGCTTTGGCTTGGGGTAACCCGGCCGAAGGTTTTAAACACAAAGATTTTATCTTTTTCTTTTAAACATCAGTCAACGAAGTTTTAAACTATAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCAACGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGGTTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCCCTCGGGTTTGGTATTGAGTGATACTCTGTCCAGGGTTAACTTGAAATAGTGAATTGGCAAGAGTGTGCTTCTGCGGCCTCTTGACTGAGATAATGTATTAGGTTCTACCAACTCGTTATAGCGGTCTAGAAAGTCCAGGCGGCATATTGAGCTCGGCCCAACAACCACCATAAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAG

>D1D2 region of the 28S ribosomal RNA gene; GenBank number JQ906764:

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCACCCTCGGTGCCCGAGTTGTAATTTGAAGATAGTTTTCTGGCACTGGCCCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTCTGGCGGGGAGTCCAGTGCTTTGTAGATTTCTATCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTATTCGATCAGACTTGGTGTTTTGTGATTATCTTCCCTTCTTGGGTTGTGCACTCGCATTTCACTGGGCCAGCATCGGTTCGGGTGGCAAGATAATGACATTGGAACGTGGCACCTTCTTCGGATGGTGTGTTATAGCCCTTGTTGATGTTGCCTGCCTGGACCGAGGACTGCGGCTTCTGCCTAGGATGCTGGCGTAATGATCCAATACCG

>18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AAGGATCATTAAAGTATTCTTCAGGCAGCCAGCGCTTCCACAGCGCGGCCGCCTAAACCTTACACACTGTGATTAGTTTTTTTATCATTGCTTTGGCTTGGGGTAACCCGGCCGAAGGTTTTAAACACAAAGATTTTATCTTTTTCTTTTAAACATCAGTCAACGAAGTTTTAAACTATAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCAACGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGGTTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCCCTCGGGTTTGGTATTGAGTGATACTCTGTCCAGGGTTAACTTGAAATAGTGAATTGGCAAGAGTGTGCTTCTGCGGCCTCTTGACTGAGATAATGTATTAGGTTCTACCAACTCGTTATAGCGGTCTAGAAAGTCCAGGCGGCATATTGAGCTCGGCCCAACAACCACCATAAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAG

>D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCACCCTCGGTGCCCGAGTTGTAATTTGAAGATAGTTTTCTGGCACTGGCCCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTCTGGCGGGGAGTCCAGTGCTTTGTAGATTTCTATCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTATTCGATCAGACTTGGTGTTTTGTGATTATCTTCCCTTCTTGGGTTGTGCACTCGCATTTCACTGGGCCAGCATCGGTTCGGGTGGCAAGATAATGACATTGGAACGTGGCACCTTCTTCGGATGGTGTGTTATAGCCCTTGTTGATGTTGCCTGCCTGGACCGAGGACTGCGGCTTCTGCCTAGGATGCTGGCGTAATGATCCAATACCG

Morphology

On YM medium at 25C after 7 days, colonies creamy white to beige, smooth, with entire margin. Cells globose to ellipsoidal, smooth, small cells 3-3.75µm X 1.5-3µm, large cells 4.5-7.5µm X 4.5-6µm. Pseudohyphae present.

Name of Depositor	CF Lee
Special Collection	ATCC
Chain of Custody	ATCC <-- CF Lee
Isolation	Soil, Sinyi, Nantou, Taiwan
Cross References	Nucleotide (GenBank) : JQ906769 ITS including 5.8S rRNA gene Nucleotide (GenBank) : JQ906764 D1/D2 region of 28S rRNA gene
References	Chang CF, et al. Five novel species of the anamorphic genus Candida in the Cyberlindnera clade isolated from natural substrates in Taiwan. Antonie van Leeuwenhoek 102: 9-21, 2012. PubMed: 22331448

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

Candida takata Lee (ATCC® MYA-4702™)

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Candida takata Lee (ATCC^® MYA-4702^™)