SUP-T1 [VB] (ATCC® CRL-1942)

Organism: Homo sapiens, human  /  Cell Type: T lymphoblast  /  Disease: T-cell lymphoblastic lymphoma

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Organism Homo sapiens, human
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease T-cell lymphoblastic lymphoma
Age 8 years
Gender male
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype 92, XXYY, -2, -2, -9, -9, the following markers are present: 2inv(2*) (p21q11), +2inv(2*), del(2*)(Q21), 2del(4)(q31q35), 2del(5)(p13p14), 2del(6)(q23q27), 2inv(14)(q11q32), t(7;9)(q34;q34), der(9)t(7;9)
Images CRL-1942 Micrograph
Derivation
This line was derived from malignant cells collected from the malignant pleural effusion of an 8 year old child with T-Cell Lymphoblastic Lymphoma.
Clinical Data
8 years
Caucasian
male
Antigen Expression

Leu-6 (CD1a) +; Leu-4 (CD3) +; Leu-3 (CD4) +; Leu-1 (CD5) +; Leu-9 (CD7) +; Leu-2a (CD8) +; OKT-10 (CD38) + RefSmith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672 RefHecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800 RefLifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463 

Genes Expressed
immunoglobulin (cytoplasmic)
Cellular Products
immunoglobulin (cytoplasmic)
Comments
The cells express multiple T lineage markers.
The cells are E - rosette negative and beta 2 microglobulin positive.
The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) and Tac (CD25).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 x 105 viable cells/mL and maintain between 2 x105 and 2 x 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor SD Smith
References

Smith SD, et al. Clinical and biologic characterization of T-cell neoplasias with rearrangements of chromosome 7 band q34. Blood 71: 395-402, 1988. PubMed: 2962650

Baer R, et al. Fusion of an immunoglobulin variable gene and a T cell receptor constant gene in the chromosome 14 inversion associated with T cell tumors. Cell 43: 705-713, 1985. PubMed: 3935328

Reynolds TC, et al. Analysis of DNA surrounding the breakpoints of chromosomal translocations involving the beta T cell receptor gene in human lymphoblastic neoplasms. Cell 50: 107-117, 1987. PubMed: 3036364

Smith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672

Hecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800

Lifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Smith SD, et al. Clinical and biologic characterization of T-cell neoplasias with rearrangements of chromosome 7 band q34. Blood 71: 395-402, 1988. PubMed: 2962650

Baer R, et al. Fusion of an immunoglobulin variable gene and a T cell receptor constant gene in the chromosome 14 inversion associated with T cell tumors. Cell 43: 705-713, 1985. PubMed: 3935328

Reynolds TC, et al. Analysis of DNA surrounding the breakpoints of chromosomal translocations involving the beta T cell receptor gene in human lymphoblastic neoplasms. Cell 50: 107-117, 1987. PubMed: 3036364

Smith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672

Hecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800

Lifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463