Toledo (ATCC® CRL-2631)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: peripheral blood  /  Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Age adult
Gender female
Ethnicity White
Applications
This cell line is a model system for studying non-Hodgkin lymphomas.
Storage Conditions liquid nitrogen vapor phase
Derivation
Toledo was established in 1990 from peripheral blood leukocytes (PBL) of a patient that originally had a diffuse large cell lymphoma (DLCL).
Clinical Data
adult
female
White
Antigen Expression
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 -
Comments
Following high-dose chemotherapy and bone marrow transplantation, the patient subsequently developed a lymphoma in the brain.
Though the morphology of the cells resembles Burkitt's lymphoma, the cells lack the typical chromosomal translocations of Burkitt's lymphoma. The karyotype does exhibit multiple chromosomal aberrations.
The cells do not express surface or cytoplasmic immunoglobulin.
The cells are negative for Epstein-Barr virus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
D5S818:10,12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8,11
vWA: 16,17
Amelogenin: X
CSF1PO: 12
D13S317:11
D16S539:9
Population Doubling Time 24 to 30 hrs
Name of Depositor H Ben-Bassat
Year of Origin 1990
References

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273