KM201 (ATCC® TIB-240)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits View Restrictions

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
KM201 recognizes an epitope close to, but distinct from, those detected by KM81 and KM114 (see ATCC TIB-241 and ATCC TIB-242).
Derivation
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44)
Cellular Products
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44)
Comments
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody blocks adhesion of a B cell hybridoma (BM-2) to BMS2 cells or to hyaluronate coated dishes.
The antibody prevents growth of lymphoid and myeloid cells in long term bone marrow cultures.
KM201 recognizes an epitope close to, but distinct from, those detected by KM81 and KM114 (see ATCC TIB-241 and ATCC TIB-242).
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor PW Kincade
References

Miyake K, et al. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J. Exp. Med. 171: 477-488, 1990. PubMed: 2406365

Miyake K, et al. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J. Exp. Med. 172: 69-75, 1990. PubMed: 2193100

Yu Q, Toole BP. A new alternatively spliced exon between v9 and v10 provides a molecular basis for synthesis of soluble CD44. J. Biol. Chem. 271: 20603-20607, 1996. PubMed: 8702806

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

1. This hybridoma is provided for research purposes only. Neither the cell line nor the antibody derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. 2. Any proposed commercial use of these cells or antibody produced from them must first be negotiated with the Oklahoma Medical Research Foundation, 825 N.E. 13th, Oklahoma City, OK 73104. Telephone (405) 271-7905.

References

Miyake K, et al. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J. Exp. Med. 171: 477-488, 1990. PubMed: 2406365

Miyake K, et al. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J. Exp. Med. 172: 69-75, 1990. PubMed: 2193100

Yu Q, Toole BP. A new alternatively spliced exon between v9 and v10 provides a molecular basis for synthesis of soluble CD44. J. Biol. Chem. 271: 20603-20607, 1996. PubMed: 8702806