FHM (ATCC® CCL-42)

Organism: Pimephales promelas, fathead minnow  /  Cell Type: epithelial  /  Tissue: connective tissue and muscle  / 

Organism Pimephales promelas, fathead minnow
Tissue connective tissue and muscle
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Storage Conditions liquid nitrogen vapor phase
Derivation
The FHM (Fat Head Minnow) line of epithelial cells was derived from tissue posterior to the anus, exclusive of the caudal fin, of normal adult minnows of both sexes by M. Gravell and R.G. Malsberger, in April, 1962.
Virus Susceptibility Infectious pancreatic necrosis virus
Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana)
Virus Resistance
poliovirus 1
Comments The cells multiply over a wide range of temperatures (0 to 36°C) with optimal growth between 28°C and 34°C.
Complete Growth Medium Minimum essential medium (Eagle) in Hanks' BSS, 90%; fetal bovine serum, 10%
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and  allow the flask to sit at room temperature. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. 
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
  6. Incubate cultures at 34°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: At the time of subcultivation (this will vary depending upon the temperature of incubation)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 34°C; (Max. 34°C, Min. 28°C)
Name of Depositor RG Malsberger
Year of Origin April, 1962
References

Gravell M, Malsberger RG. A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci. 126: 555-565, 1965. PubMed: 5220177

Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. Hyg. 78: 173-180, 1963. PubMed: 14063721

Basic Documentation
References

Gravell M, Malsberger RG. A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci. 126: 555-565, 1965. PubMed: 5220177

Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. Hyg. 78: 173-180, 1963. PubMed: 14063721