Description of cloned agent:
Strain: H388, variant #16
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from peripheral blood mononuclear cell DNA of animal H388 by J. Lednicky.
Plasmid size: 7893 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5207 bp]. High-copy number; pUC-19 has a ColEI ori.
VRMC-11 (variant #16) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-9 and VRMC-10).
Plasmid might dimerize; restriction pattern will not change.
Restriction enzyme analysis: BamHI--4.479,3.414; EcoRI--5.207,2.686; KpnI--6.392,1.501; SalI--7.893; SfiI--7.893; XhoI--uncut.
Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by: Xian Min Dai. Clone viability: not tested.