Simian virus 40 (ATCC® VRMC-11)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

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Designations pUCSVH388-16 [molecular clone of Simian virus 40, SV-40]
Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Isolation
Description of cloned agent:
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from peripheral blood mononuclear cell DNA of animal H388 by J. Lednicky.
Applications
contains sequence
Vector
Construct size (kb): 7.89300012588501

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector:  pUC-19

        Intact vector size (kb):  2.686 kb

Insert
DNA: genomic
Insert lengths(kb): 5.206999778747559
Tissue: monkey peripheral blood mononuclear cell
Insert Size (kb) 5.207
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Description of cloned agent:
Name: SV40
Strain: H388, variant #16
Classification: Papovavirus
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from peripheral blood mononuclear cell DNA of animal H388 by J. Lednicky.
Plasmid size: 7893 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5207 bp]. High-copy number; pUC-19 has a ColEI ori.
VRMC-11 (variant #16) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-9 and VRMC-10).
Plasmid might dimerize; restriction pattern will not change.
Restriction enzyme analysis: BamHI--4.479,3.414; EcoRI--5.207,2.686; KpnI--6.392,1.501; SalI--7.893; SfiI--7.893; XhoI--uncut.
Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by: Xian Min Dai. Clone viability: not tested.
Classification Polyomaviridae, Polyomavirus
References

Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685

John A Lednicky, personal communication

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