Cell Line Description:
Epithelial cells from a histologically normal adult human prostate were isolated and subsequently transfected with a plasmid carrying one copy of the human papillomavirus 18 (HPV-18) genome to establish the RWPE-1 (ATCC CRL-11609) cell line.
Cells from the RWPE-1 cell line were further transformed by Ki-ras using the Kirsten murine sarcoma virus (Ki-MSV) to establish the RWPE-2 cell line (ATCC CRL-11610). RWPE-2 cells form colonies in agar and tumors in nude mice.
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Quality Control Specifications
DNA Quality Control Information:
· > 100 kb in size by agarose gel electrophoresis
· No RNA detected in the agarose gel
· A260/A280 ratio ³ 1.8
· Tested and verified for PCR amplification and restriction digestion.