JVM-2 (ATCC® CRL-3002)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed  /  Tissue: peripheral blood  /  Disease: Mantle Cell Lymphoma

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2
Disease Mantle Cell Lymphoma
Age 63
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Bimodal chromosome number: 46 and 92This is a bimodal female cell line with approximately half the cells being pseudodiploid and the other half pseudotetraploid. Most cells contain the t(11;14)(q13;q32) derivative chromosome common to Mantle Cell Lymphoma. Other derivative chromosomes included: t(3;8)(q13;p21), add(4q) [or t(4;13)(q2?5;q1?4)], del(13)(q21) and dup(16p).
Images
Derivation
Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA.
Clinical Data
63
Caucasian
female
Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA. JVM-2 is characterized cytogenetically by t(11;14)(q13; q32) [PubMed: 3093393 and 3262465].
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+ (verified at ATCC)
Oncogene p53 [PubMed: 16434365]
Genes Expressed
p53 RefSalaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365,CD3-, CD5-, CD10-, CD19+, CD20+, CD23+ (verified at ATCC)
Comments

JVM-2 is characterized cytogenetically by t(11;14)(q13; q32) RefMelo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393 RefMelo JV, et al. The establishment of cell lines from chronic B cell leukaemias: evidence of leukaemic origen by karyotypic abnormalities and Ig gene rearrangement. Clin. Exp. Immunol. 73: 23-28, 1988. PubMed: 3262465. JVM-2 cells retain p16 expression and exhibit low expression of cyclin D1. This cell line expresses cyclin D2 RefTucker CA, et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk. Res. 30: 449-457, 2006. PubMed: 16183118. Based on the new classification of lymphomas, JVM-2 is considered a Mantle Cell Lymphoma (MCL) RefTucker CA, et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk. Res. 30: 449-457, 2006. PubMed: 16183118 Refde Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187. .

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 104 to 3 x 104 viable cells/mL. Do not allow the cell concentration to exceed 1 X 106 cells/mL.
Cryopreservation
70% growth medium; 20% FBS; 10% DMSO
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17
Population Doubling Time about 48 hours
Name of Depositor J.V.Melo
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

de Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187.

Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.

Basic Documentation
Other Documentation
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

de Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187.

Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.