D341 Med (ATCC® HTB-187)

Organism: Homo sapiens, human  /  Cell Type: Medulloblastoma  /  Tissue: brain/cerebellum  /  Disease: medulloblastoma

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Organism Homo sapiens, human
Tissue
brain/cerebellum
Cell Type Medulloblastoma
Product Format frozen
Morphology spheroid
Culture Properties suspension
Biosafety Level 1
Disease medulloblastoma
Age 3.5 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are: 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy.
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Derivation
The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.
Clinical Data
The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.
male
Genes Expressed
The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.
Cellular Products
neurofilament proteins
Tumorigenic Yes
Effects
Yes, in nude mice; forms serially transplantable intercranial and subcutaneous tumors
Yes, in soft agar
Comments
The cells produce tumors in nude mice.
The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.
The c-myc oncogene is amplified in D341 Med cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 105 viable cells/mL. Maintain cultures between 4 X 105 and 1 X 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,13
D16S539: 12,14
D5S818: 11,12
D7S820: 9,13
F13A01: 6,7
F13B: 8,10
FESFPS: 10,13
LPL: 11
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18
Population Doubling Time 37 hrs
Name of Depositor HS Friedman
Year of Origin 1988
References

Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793

He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793

He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024