pSM17 (53353)

Applications: expression vectorshuttle vector  /  Depositors: G Grandi

Permits and Restrictions

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Designations pSM17
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors G Grandi
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Bacillus subtilis SMS108
Vector Information
Size (kb): 8.1000003814697270
Vector: pSM17 (plasmid)
Promoters: Promoter ErmC
Construction: pSM6, pSM100
Marker(s):kanR
Construct size (kb): 8.100000381469727
Features: marker(s): kanR
promoter: ErmC
replicon: pUB110, pE194
Applications
expression vector
shuttle vector
Comments
The EcoRI cloning site is 4 bp downstream of the ribosome binding sequence.
pBR322 sequences were removed from pSM6 (ATCC 53352) by PstI digestion and replaced with pSM100 (pUB110 modified to remove the EcoRI site).
Media ATCC® Medium 1638: LB medium (ATCC medium 1065) with 5 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Grandi G, et al. New plasmid expression vectors for Bacillus subtilis. Plasmid 16: 1-14, 1986. PubMed: 3090575

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
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