pSM17 (ATCC® 53353)

Applications: expression vectorshuttle vector  /  Depositors: G Grandi

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Designations pSM17
Depositors G Grandi
Biosafety Level 1
Host
Distribution host: Bacillus subtilis SMS108
Vector Information
Size (kb): 8.1000003814697270
Vector: pSM17 (plasmid)
Promoters: Promoter ErmC
Construction: pSM6, pSM100
Marker(s):kanR
Construct size (kb): 8.100000381469727
Features: marker(s): kanR
promoter: ErmC
replicon: pUB110, pE194
Applications
expression vector
shuttle vector
Comments
The EcoRI cloning site is 4 bp downstream of the ribosome binding sequence.
pBR322 sequences were removed from pSM6 (ATCC 53352) by PstI digestion and replaced with pSM100 (pUB110 modified to remove the EcoRI site).
Media ATCC® Medium 1638: LB medium (ATCC medium 1065) with 5 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Grandi G, et al. New plasmid expression vectors for Bacillus subtilis. Plasmid 16: 1-14, 1986. PubMed: 3090575

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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