M-NFS-60 (ATCC® CRL-1838)

Organism: Mus musculus, mouse  /  Cell Type: virus induced  /  Disease: myelogenous leukemia

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Organism Mus musculus, mouse
Cell Type virus induced
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease myelogenous leukemia
Applications
This cell line is a suitable transfection host.
Derivation
The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus.
Comments
The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF).
The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • 62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF)
  • fetal bovine serum to a final concentration of 10%.
  • Subculturing
    Cultures can be maintained by addition or replacement of fresh medium. Subculture every two days at 2.5 X 104 viable cells/mL.
    Medium Renewal: Add fresh medium at the time of subculture
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37°C
    Name of Depositor JF Weaver, A Creasey
    References

    Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

    Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

    Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527