GK1.5 (ATCC® TIB-207)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: Hybridoma: B lymphocyte  /  Tissue: Spleen  / 

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue Spleen
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1
Strain Lewis (B cell); BALB/c (myeloma)
Applications
The antibody profoundly blocks antigen specific murine class II MHC antigen reactive helper T lymphocyte lines.
Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with the cloned cytotoxic T lymphocyte lines V4 and 243/2.5.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against mouse helper, inducer T cells (L3T4 antigen, CD4)
Cellular Products immunoglobulin; monoclonal antibody; against mouse helper, inducer T cells (L3T4 antigen, CD4)
Comments
L3T4 is expressed on mouse helper/inducer T cells and is analogous to the human Leu-3/T4 molecule.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL. Do not allow the cell concentration to exceed 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype Rat IgG2b
Name of Depositor FW Fitch
References

Fitch FW, et al. Evidence implicating L3T4 class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J. Immunol. 131: 2178-2183, 1983. PubMed: 6195255

Dialynas DP, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J. Immunol. 131: 2445-2451, 1983. PubMed: 6415170

Garvy BA, Harmsen AG. The role of T cells in infection-driven interstitial pneumonia after bone marrow transplantation in mice. Transplantation 62: 517-525, 1996. PubMed: 8781619

Murray HW, et al. Models of relapse of experimental visceral leishmaniasis. J. Infect. Dis. 173: 1041-1043, 1996. PubMed: 8603949

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Murray HW, et al. Multiple host defense defects in failure of C57BL/6 ep/ep (Pale Ear) mice to resolve visceral Leishmania donovani infection. Infect. Immun. 64: 161-166, 1996. PubMed: 8557335

Dialynas DP, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol. Rev. 74: 29-56, 1983. PubMed: 6195085

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Basic Documentation
References

Fitch FW, et al. Evidence implicating L3T4 class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J. Immunol. 131: 2178-2183, 1983. PubMed: 6195255

Dialynas DP, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J. Immunol. 131: 2445-2451, 1983. PubMed: 6415170

Garvy BA, Harmsen AG. The role of T cells in infection-driven interstitial pneumonia after bone marrow transplantation in mice. Transplantation 62: 517-525, 1996. PubMed: 8781619

Murray HW, et al. Models of relapse of experimental visceral leishmaniasis. J. Infect. Dis. 173: 1041-1043, 1996. PubMed: 8603949

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Murray HW, et al. Multiple host defense defects in failure of C57BL/6 ep/ep (Pale Ear) mice to resolve visceral Leishmania donovani infection. Infect. Immun. 64: 161-166, 1996. PubMed: 8557335

Dialynas DP, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol. Rev. 74: 29-56, 1983. PubMed: 6195085

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm