pBSL193 (ATCC® 87152)

Applications: contains easily purifiable cassette(s) for construction omega-tet  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL193
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.2750000953674320
Vector: pBSL193 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,tetR
Construct size (kb): 5.275000095367432
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: tetR
Applications
contains easily purifiable cassette(s) for construction omega-tet
Comments
Restriction digests of the clone give the following sizes (kb):BamHI--3.0, 2.3; EcoRI--3.0, 1.45, 0.6, 0.4; KpnI--3.0, 2.3; MluI--3.15, 2.2; SstI--3.0, 2.35.
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Tc element flanked by multiple cloning sites duplicated in tandem orientation.
Corresponds to the EcoRI/StyI fragment of pBR322, with BamHI, SalI, AccI, HincII, and HindIII sites removed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
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