32.2 (ATCC® HB-9469)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody is specific for the 72000 dalton, high affinity Fc receptor on human monocytes.
Derivation
Animals were immunized with partially purified FcR from U937 cells.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human monocyte Fc receptor (high affinity, FcRI), CD64
Cellular Products
immunoglobulin; monoclonal antibody; against human monocyte Fc receptor (high affinity, FcRI), CD64
Comments
Animals were immunized with partially purified FcR from U937 cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody is specific for the 72000 dalton, high affinity Fc receptor on human monocytes.
Binding of the antibody is not inhibited by human IgG, and the antibody does not block human IgG binding to the receptor.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 6 mM HEPES and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Trustees of Dartmouth College
References

Graziano RF, Fanger MW. FcgammaRI and FcgammaRII on monocytes and granulocytes are cytotoxic trigger molecules for tumor cells. J. Immunol. 139: 3536-3541, 1987. PubMed: 2960735

Petroni KC, et al. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-gamma and glucocorticoids. J. Immunol. 140: 3467-3472, 1988. PubMed: 2966197

Fanger MW, et al. Monoclonal antibodies to FC receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 4,954,617 dated Sep 4 1990

Fanger MW, et al. Cytotoxicity mediated by human Fc receptors for IgG. Immunol. Today 10: 92-99, 1989. PubMed: 2525910

Shen L, et al. Polymorphonuclear leukocyte function triggered through the high affinity Fc receptor for monomeric IgG. J. Immunol. 139: 534-538, 1987. PubMed: 2955043

Shen L, et al. Heteroantibody-mediated cytotoxicity: antibody to the high affinity Fc receptor for IgG mediates cytotoxicity by human monocytes that is enhanced by interferon-gamma and is not blocked by human IgG. J. Immunol. 137: 3378-3382, 1986. PubMed: 2946759

Anderson CL, et al. Monoclonal antibodies to Fc receptors for IgG on human mononuclear phagocytes. Antibody characterization and induction of superoxide production in a monocyte cell line. J. Biol. Chem. 261: 12856-12864, 1986. PubMed: 3017990

Ball ED, Fanger MW. Bispecific molecules for use in inducing antibody dependent effector cell-mediated cytotoxicity. US Patent 5,833,985 dated Nov 10 1998

Tempest PR, et al. Humanized antibodies to Fc receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 6,500,931 dated Dec 31 2002

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Graziano RF, Fanger MW. FcgammaRI and FcgammaRII on monocytes and granulocytes are cytotoxic trigger molecules for tumor cells. J. Immunol. 139: 3536-3541, 1987. PubMed: 2960735

Petroni KC, et al. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-gamma and glucocorticoids. J. Immunol. 140: 3467-3472, 1988. PubMed: 2966197

Fanger MW, et al. Monoclonal antibodies to FC receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 4,954,617 dated Sep 4 1990

Fanger MW, et al. Cytotoxicity mediated by human Fc receptors for IgG. Immunol. Today 10: 92-99, 1989. PubMed: 2525910

Shen L, et al. Polymorphonuclear leukocyte function triggered through the high affinity Fc receptor for monomeric IgG. J. Immunol. 139: 534-538, 1987. PubMed: 2955043

Shen L, et al. Heteroantibody-mediated cytotoxicity: antibody to the high affinity Fc receptor for IgG mediates cytotoxicity by human monocytes that is enhanced by interferon-gamma and is not blocked by human IgG. J. Immunol. 137: 3378-3382, 1986. PubMed: 2946759

Anderson CL, et al. Monoclonal antibodies to Fc receptors for IgG on human mononuclear phagocytes. Antibody characterization and induction of superoxide production in a monocyte cell line. J. Biol. Chem. 261: 12856-12864, 1986. PubMed: 3017990

Ball ED, Fanger MW. Bispecific molecules for use in inducing antibody dependent effector cell-mediated cytotoxicity. US Patent 5,833,985 dated Nov 10 1998

Tempest PR, et al. Humanized antibodies to Fc receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 6,500,931 dated Dec 31 2002