Sl/Sl4 is a SV40 large T antigen immortalized stromal cell line derived from the hematopoietic microenvironment of a fetal murine homozygous (Sl/Sl) SCF-deficient embryo.
Defects in the hematopoietic microenvironment, associated with the Steel (Sl) mutation in
mice, have been shown to be due to abnormalities in the production or presentation of the protein product of the Steel gene.
This product is termed stem cell factor (SCF) or mast cell growth factor (MGF). It exists as a locally secreted or membrane-bound protein.
Stable stromal cell transfectants can differentially process the two forms of human SCF protein product, membrane-bound and secreted SCF.
Membrane-bound SCF is available in the transfected Sl/Sl4 cell line, Sl/Sl4 hSCF220 (ATCC CRL-2453).
Secreted SCF is available in the transfected Sl/Sl4 cell line, Sl/Sl4 hSCF248 (ATCC CRL-2454)