pRS423 (ATCC® 77104)

Applications: YE-type (episomal) shuttle vectorvector containing primer sites useful for sequencingvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: TW Christianson

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Designations pRS423
Depositors TW Christianson
Biosafety Level 1
Vector Information
Size (kb): 5.797
Vector: pRS423 (phagemid)
Promoters: Promoter T7 (phi10)
Construction: pJS92, pRS303, pBluescript II SK+
Marker(s):ampR,HIS3
Construct size (kb): 5.797
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: T3
promoter: T7 (phi10)
promoter: lac
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
Applications
YE-type (episomal) shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PvuI--3.7, 2.1.
Contains the REP3 and FRT sequences necessary for high copy propagation in yeast.
In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.3 + or - 1.3% of progeny per doubling.
YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.
The beta-galactosidase alpha peptide permits blue-white detection of recombinants.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568

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Shipping Information Freeze dried phagemid in E. coli