KHOS-312H (ATCC® CRL-1546)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: osteosarcoma

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Organism Homo sapiens, human
Tissue bone
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease osteosarcoma
Age 13 years
Gender female
Ethnicity Caucasian
Clinical Data
female
Caucasian
13 years
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
The growth properties of KHOS-312H are similar to HOS (ATCC CRL-1543).
This line is a revertant of ATCC CRL-1544 that no longer has a rescuable Kirsten murine sarcoma virus genome.

Note: An inconsistency in our records was discovered late in 2010 concerning the human osteosarcoma cell line, KHOS-312H. Due to an ATCC catalog transfer error, this cell line has been referred to as both KHOS-312H and KHOS-321H by ATCC and in the literature. The name originally supplied to ATCC by the depositor was KHOS-312H.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Remove medium, rinse with fresh 0.25% trypsin-0.53 mM EDTAsolution, remove trypsin and let the culture sit at 37°C for 10 to 12 minutes or until cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 days.

Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal:
2 to 3 times per week


STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Name of Depositor JS Rhim
References

Yang YH, et al. Reversion of Kirsten sarcoma virus transformed human cells: elimination of the sarcoma virus nucleotide sequences. J. Gen. Virol. 43: 447-451, 1979. PubMed: 225428

Cho HY, et al. Revertants of human cells transformed by murine sarcoma virus. Science 194: 951-953, 1976. PubMed: 62397

Cho HY, Rhim JS. Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen. Science 205: 691-693, 1979. PubMed: 223242

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Yang YH, et al. Reversion of Kirsten sarcoma virus transformed human cells: elimination of the sarcoma virus nucleotide sequences. J. Gen. Virol. 43: 447-451, 1979. PubMed: 225428

Cho HY, et al. Revertants of human cells transformed by murine sarcoma virus. Science 194: 951-953, 1976. PubMed: 62397

Cho HY, Rhim JS. Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen. Science 205: 691-693, 1979. PubMed: 223242