ARPE-19/HPV-16 (ATCC® CRL-2502)

Organism: Homo sapiens, human  /  Cell Type: human papillomavirus 16 (HPV-16) transfected  / 

Organism Homo sapiens, human
Cell Type human papillomavirus 16 (HPV-16) transfected
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain Human Papilloma viral 16 (HPV16) sequences
Age 19 years
Gender male
Applications
transfection host
Karyotype diploid
Derivation
The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302) by transfection with DH5-HPV-16.
ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident.
Clinical Data
ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident.
male
Antigen Expression
RPE-specific markers CRALBP and RPE-65
Genes Expressed
RPE-specific markers CRALBP and RPE-65
Comments
The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302) by transfection with DH5-HPV-16.
ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident.
The cells express the RPE-specific markers CRALBP and RPE-65.
Complete Growth Medium The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to culture vessels.
  7. Incubate cultures at 37C.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X, Y
CSF1PO: 11
D13S317: 11, 12
D16S539: 9, 11
D5S818: 13
D7S820: 9, 11
THO1: 6, 9.3
TPOX: 9, 11
vWA: 16, 19
Name of Depositor JW Obringer
References

Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076

Basic Documentation
References

Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076