Influenza B virus (ATCC® VR-1784)

Classification: Orthomyxoviridae, Influenzavirus B  /  Product Format: 1 mL per vial

Classification Orthomyxoviridae, Influenzavirus B
Agent Influenza B virus
Strain B/Virginia/ATCC4/2009
Biosafety Level 2
Product Format 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1784 images
Comments This item was isolated in cell culture by four passages in MDCK cells.
Effect on Host CPE, cell rounding and sloughing
Recommended Host MDCK (ATCC® CCL-34™)
Growth Conditions Temperature: 35°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.

Incubation: 2-3 days
Effect on Host CPE, cell rounding and sloughing
Name of Depositor ATCC
Source Specimen from a clinical patient in Virginia, 2009