Clostridium difficile (Hall and O'Toole) Prevot (ATCC® BAA-1803)

Type Strain: no  /  Biosafety Level: 2

Application
Enteric Research
Isolation
Clinical specimen
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Preceptrol® no
Genotype Toxinotype: IIIc;
Type Strain no
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Confirmed by CDC: NAP1, Toxinotype III, binary toxin positive
Binary Toxin gene cdtB was amplified by PCR
Toxinotype IIIc
Ribotype 027
Medium Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Genotype Toxinotype: IIIc;
Name of Depositor R Clark
Isolation
Clinical specimen
References

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Stubbs, S et al. Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol. Lett. 186(2): 307-312, 2000. PubMed: 10802189

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986