pRB382 (ATCC® 77378)

Applications: expression vectorshuttle vectorvector permitting visual detection of activity in colonies  /  Depositors: R Bruckner

Permits and Restrictions

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Designations pRB382
Depositors R Bruckner
Biosafety Level 1
Vector Information
Size (kb): 8.900
Vector: pRB382 (plasmid)
Promoters: Promoter vegII (B. subtilis)
Construction: pUB110, pBR322, lacZ, vegII
Marker(s): ampR,bleR,neoR
Construct size (kb): 8.900
Features: marker(s): ampR
marker(s): bleR
marker(s): neoR
promoter: vegII (B. subtilis)
replicon: pMB1
replicon: pUB110
reporter group: 'lacZ
MCS: HindIII...BamHI
terminator: To phage lambda
transcription terminator: rrnB
Applications
expression vector
shuttle vector
vector permitting visual detection of activity in colonies
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.5; EcoRI/BglI--5.1, 2.2, 1.1, 0.8; BglI/BglII--3.5, 2.2, 2.0, 1.4.
The Bacillus subtilis promoter vegII initiates transcription in both B. subtilis and E. coli.
Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media.
May not be suitable for cloning very strong expression signals.
Translational fusion shuttle vector using 'lacZ as reporter. Also contains the vegII promoter. Useful for construction of fusion proteins.
Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin.
The 5' truncated 'lacZ gene, lacking transcriptional and translational initiation signals, is useful in both E. coli and in B. subtilis, since the endogenous beta-galactosidase level in B. subtilis is low.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028

Related Products
Component of: ATCC 87056
Shipped frozen
Product Sheet
Product Sheet
Product Sheet
Attachments ATCC 77378 Vector Map