YIp5-KanR (ATCC® 37473)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: CA Reddy

Permits and Restrictions

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Designations YIp5-KanR
Depositors CA Reddy
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipped in: E. coli C600
Vector Information
Size (kb): 7.0999999046325680
Vector: YIp5-KanR (plasmid)
Construction: kanR of pNG35 into PvuII site of YIp5
Construct size (kb): 7.099999904632568
Features: marker(s): ampR, kanR, tetR, URA3
replicon: pMB1
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Vector provides selection markers for transformation of Saccharomyces cerevisiae or other eukaryotes based on G418 resistance.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Rao TR, Reddy CA. A YIp5-kanR plasmid useful for isolating ars from yeast and other eukaryotes based on G418 resistance selection. Nucleic Acids Res. 14: 7504, 1986. PubMed: 3532035

Struhl K, et al. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1979. PubMed: 375221

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried plasmid in E. coli