Influenza A virus (H3N2) (ATCC® VR-1680)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

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Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H3N2)
Strain A/Aichi/2/68
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
Derived by adaptation from allantoic product ATCC® VR-547™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-547™ and ATCC® VR-1680™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture.
Effect on Host
CPE, rounding and degeneration
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, rounding and degeneration
Name of Depositor MT Coleman, Centers for Disease Control and Prevention; ATCC®
Source Isolated from sailor on Israeli ship docking in Aichi, Japan, 1968
References

Coleman MT, et al. The Hong Kong-68 influenza A2 variant. Lancet 2: 1384-1386, 1968. PubMed: 4177941

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation