HK-PEG-1 [formerly PEG 1-6] (ATCC® CCL-189)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Influenza

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Influenza
Applications
The line was produced by fusing P3X63Ag8 myeloma cells with spleen cells from BALB/c mice that had been immunized with influenza virus (A.PR/8/34).
The hybridoma cell line HK-PEG-1 secretes a mouse monoclonal antibody (IgG3) that reacts with influenza virus.
Storage Conditions liquid nitrogen vapor phase
Derivation
The hybridoma cell line HK-PEG-1 secretes a mouse monoclonal antibody (IgG3) that reacts with influenza virus. The line was produced by fusing P3X63Ag8 myeloma cells with spleen cells from BALB/c mice that had been immunized with influenza virus (A.PR/8/34).
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
The hybridoma cell line HK-PEG-1 secretes a mouse monoclonal antibody (IgG3) that reacts with influenza virus. The line was produced by fusing P3X63Ag8 myeloma cells with spleen cells from BALB/c mice that had been immunized with influenza virus (A.PR/8/34).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 to 8 X 10(4) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium (depending on cell density) every 2-3 days.
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG3
Name of Depositor WU Gerhard
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation