CHLA-04-ATRT (ATCC® CRL-3036)

Organism: Homo sapiens, human  /  Tissue: Brain (lateral and third ventricle)  /  Disease: Atypical Teratoid Rhabdoid Tumor (ATRT)

Organism Homo sapiens, human
Tissue Brain (lateral and third ventricle)
Morphology rounded
Culture Properties single cells and clusters in suspension
Biosafety Level 1
Disease Atypical Teratoid Rhabdoid Tumor (ATRT)
Age 20 months
Gender Male
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human diploid cell line of male origin in which most of the examined cells contained the karyotypically normal male chromosome complement, 46,XY.
Images CRL-3036 Micrograph
Derivation CHLA-04-ATRT was established from a 20 month old boy with recurrent atypical teratoid rhabdoid tumor (ATRT) with wide dissemination in the brain and CSF.  Cells were isolated from the brain tumor at the time of autopsy and were cultured in neurobasal medium after mechanical disruption.
Clinical Data 20 months old male
Antigen Expression epithelial membrane antigen (EMA1), expressed
Comments

This cell line has been continuously carried in culture for over 8 months, and in neurobasal medium grows as spheres of varying sizes.

ATCC has verified that CHLA-04-ATRT expresses epithelial membrane antigen (EMA1) and does not express INI-1.

The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Children's Cancer to Children’s Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community.

Complete Growth Medium DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
Subculturing

Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium renewal: Add fresh medium twice weekly (depending on cell density).

Cryopreservation
Freeze medium: Fetal bovine serum, 90%; DMSO, 10% (v/v).
Cell culture tested DMSO is available as ATCC 4-X
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 12
D13S317: 12
D7S820: 11
D16S539: 11
vWA: 17
TH01: 6, 9
Amelogenin: X Y
TPOX: 8
CSF1PO: 11, 13
Name of Depositor A Erdreich-Epstein, Saban Research Institute, Children’s Hospital Los Angeles