JVM-13 (ATCC® CRL-3003)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed  /  Tissue: peripheral blood  /  Disease: B-prolymphocytic leukemia (B-PLL)

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 [Cells infected with Epstein-Barr virus]
Disease B-prolymphocytic leukemia (B-PLL)
Age late 60's
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Modal chromosome number: 47This is a pseudodiploid human cell line of male origin with a modal chromosome number of 47. Most of the cells contain the derivative chromosomes: add(2q), t(8;13)(p12;q13) and t(8;?)(p12;?). Overall, the karyology was consistent with other published data [PubMed: 3262465].
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Derivation Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA.
Clinical Data
Late 60's
Caucasian
male
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7+ (verified at ATCC)
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 104 to 4 x 104 viable cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).


Cryopreservation
70% growth medium; 20% FBS; 10% DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
Population Doubling Time about 58 hours
Name of Depositor J.V.Melo
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Melo JV, et al. The establishment of cell lines from chronic B cell leukaemias: evidence of leukaemic origen by karyotypic abnormalities and Ig gene rearrangement. Clin. Exp. Immunol. 73: 23-28, 1988. PubMed: 3262465

Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Melo JV, et al. The establishment of cell lines from chronic B cell leukaemias: evidence of leukaemic origen by karyotypic abnormalities and Ig gene rearrangement. Clin. Exp. Immunol. 73: 23-28, 1988. PubMed: 3262465

Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365