XMMEN-OE5 [AAB-647, AAB647] (ATCC® HB-9081)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody neutralizes gram negative endotoxin activity, and is protective against gram negative sepsis and therapeutic for gram negative sepsis in mice and humans.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against lipopolysaccharide endotoxin of gram negative bacteria
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with killed E. coli (0111 strain, J5 mutant) cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody neutralizes gram negative endotoxin activity, and is protective against gram negative sepsis and therapeutic for gram negative sepsis in mice and humans.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype IgM
Name of Depositor Regents of the University of California
References

Young LS. Monoclonal antibodies specific for lipid-A determinants of gram negative bacteria. US Patent 4,918,163 dated Apr 17 1990

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Young LS. Monoclonal antibodies specific for lipid-A determinants of gram negative bacteria. US Patent 4,918,163 dated Apr 17 1990