Chlamydophila pneumoniae (Grayston et al.) Everett et al. (ATCC® VR-1452)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae, Chlamydophila
Agent Chlamydophila pneumoniae (Grayston et al.) Everett et al.
Strain AO3
Applications
Respiratory research
Positive for Mycoplasma Yes
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

First coronary isolate of C. pneumoniae.

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

 

Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies.
Recommended Host
HEp-2 cells (ATCC CCL-23)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies.
Name of Depositor JT Summersgill
Special Collection ATCC
Source Coronary atheroma of human with athcroslerosis
References

Ramirez JA. Isolation of Chlamydia pneumoniae from the coronary artery of a patient with coronary atherosclerosis. The Chlamydia pneumoniae/Atherosclerosis Study Group. Ann. Intern. Med. 125: 979-982, 1996. PubMed: 8967709

This holding has tested positive for Mycoplasma contamination by PCR.