pJK142 (ATCC® 86955)

Applications: expression vectorvector permitting visual detection of recombinants  /  Depositors: JB Keeney

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Designations pJK142
Depositors JB Keeney
Biosafety Level 1
Vector Information
Size (kb): 3.2720000743865970
Vector: pJK142 (plasmid)
Promoters: Promoter lac
Marker(s):ampR
Construct size (kb): 3.272000074386597
Features: insert detection: lac
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
expression vector
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.3; EcoRI--3.3; HindIII--3.3.
Expression vector permitting visual detection of recombinants by lacZalpha complementation.
The order of the major features in the plasmid is: ampR - pMB1 ori - 5' lacZ' - SacI/MCS/KpnI - 3' lacZ' - NdeI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439

Jill B Keeney, personal communication

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet