SU-DHL-4 (ATCC® CRL-2957)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: peritoneal effusion  / 

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Organism Homo sapiens, human
Tissue peritoneal effusion
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 38 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images CRL-2957 Micrograph
Clinical Data 38 years
Caucasian
male
Genes Expressed

IgG+, Kappa+, IgM-, IgA-, IgD-, Lambda-. 

This cell line has relatively high expression levels of Bax, Bak, AIF, and high caspase-9 activity.
Comments The depositor states that this cell line is negative for Epstein-Barr Virus (EBV).
This cell line has a 14;18 chromosomal translocation.
This cell line has a major rearrangement in the BCL-2 gene.
This cell line is negative for Candida ingestion.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 1.0 x 105to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium renewal: every 2 to 4 days (depending on cell density)
Cryopreservation

Storage temperature: liquid nitrogen vapor phase

Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO

Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11, 12
D13S317: 11, 12
D7S820: 8, 11
D16S539: 11, 13
vWA: 18, 19
THO1: 6, 9.3
Amelogenin: X Y
TPOX: 9, 11
CSF1PO: 12
Name of Depositor A Epstein
Year of Origin 1976
References

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412