SU-DHL-4 (ATCC® CRL-2957)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: peritoneal effusion  / 

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Organism Homo sapiens, human
Tissue peritoneal effusion
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension
Biosafety Level 1
Age 38 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images CRL-2957 Micrograph
Clinical Data 38 years
Caucasian
male
Genes Expressed

IgG+, Kappa+, IgM-, IgA-, IgD-, Lambda-. 

This cell line has relatively high expression levels of Bax, Bak, AIF, and high caspase-9 activity.
Comments The depositor states that this cell line is negative for Epstein-Barr Virus (EBV).
This cell line has a 14;18 chromosomal translocation.
This cell line has a major rearrangement in the BCL-2 gene.
This cell line is negative for Candida ingestion.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 1.0 x 105to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium renewal: every 2 to 4 days (depending on cell density)
Cryopreservation

Storage temperature: liquid nitrogen vapor phase

Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO

Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11, 12
D13S317: 11, 12
D7S820: 8, 11
D16S539: 11, 13
vWA: 18, 19
THO1: 6, 9.3
Amelogenin: X Y
TPOX: 9, 11
CSF1PO: 12
Name of Depositor A Epstein
Year of Origin 1976
References

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412