Chlamydophila pneumoniae (ATCC® VR-1310)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae, Chlamydophila
Deposited As Chlamydia pneumoniae, strain TWAR (CDC/CWL-029)
Agent Chlamydophila pneumoniae
Strain CWL-029
Applications
Respiratory research
Biosafety Level 2
[Activities with high potential for aerosol production requires Biosafety Level 3 facilities and practices.]
Product Format frozen 1 mL per vial
Storage Conditions Vapor phase of liquid nitrogen
Images VR-1310 images
Comments Please see ATCC Virology Guide for more detailed information on propagation
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Recommended Host
HEp-2 cells (ATCC® CCL-23™)
Growth Conditions Temperature: 35°C
Recommendations for Infection: For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.  Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).  Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour.  End adsorption by adding agent growth medium.
Incubation:  Incubate infected culture for 72 hours at 35°C in a humidified 5% CO2 atmosphere.
Preservation: Controlled-rate freezing.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Name of Depositor RC Barnes, Centers for Disease Control and Prevention
Special Collection NCRR Contract
Source Throat swab of pneumonia patient
References

Barnes RC, et al. Characteristics of atypical chlamydial agents isolated from pneumonia patient in Atlanta, Georgia. Annual meeting. Am. Soc. Microbiol., New Orleans, Louisiana, May 16, 1989.

Kuo CC, Grayston JT. Chlamydia spp. strain TWAR: A newly recognized organism associated with atypical pneumonia and other respiratory infections. Clin. Microbiol. Newsletter 10: 137-140, 1988.

Cross References

Nucleotide (GenBank) : AE001363.1 Chlamydophila pneumoniae CWL029, complete genome