HMCB [ Human Melanoma Cell Bowes] (ATCC® CRL-9607)

Organism: Homo sapiens, human  /  Cell Type: epithelial  /  Tissue: skin  /  Disease: melanoma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue skin
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease melanoma
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
human tissue plasminogen activator
Cellular Products
human tissue plasminogen activator
Comments
The cells originally tested positive for mycoplasma.
After treatment with ciprofloxacin, the cells have remained negative for mycoplasma for 60 days.
Complete Growth Medium Minimum essential medium (Eagle) with non-essential amino acids, 10 mM HEPES and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: culture medium 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor Genentech, Inc.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation