NCI-H510A [H510A, NCI-H510] (ATCC® HTB-184)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic tissue: adrenal gland  /  Disease: carcinoma; small cell lung cancer; extrapulmonary origin

Organism Homo sapiens, human
Tissue
lung; derived from metastatic tissue: adrenal gland
Product Format frozen
Morphology epithelial
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease carcinoma; small cell lung cancer; extrapulmonary origin
Age 56 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes were common to all cells. These included t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+. Neither DM nor HSR were detected; structurally normal N1, N2, N13 and N21 were absent. Generally, there were 3 copies of both F group chromosomes; the X chromosomes were paired, and structurally normal Y chromosome was not found.
Derivation
The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from an adrenal metastasis in an adult male patient.
Clinical Data
56 years
Caucasian
male
Tumorigenic Yes
Effects
Yes, in nude mice
Comments

The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue. 

The line does not exhibit any gross structural DNA abnormalities. 

The cells express elevated levels of four biochemical markers of SCLC: neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.

The cells form transplantable tumors with typical small cell carcinoma histology.

Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
This line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium, collecting the cells by centrifugation, resuspending in fresh medium and dispensing into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 13
D5S818: 9,12
THO1: 7,9.3
TPOX: 8
vWA: 15
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1982
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Basic Documentation
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257