MiCl1 (S+L-) (ATCC® CCL-64.1)

Organism: Mustela vison, mink  /  Disease: sarcoma

Organism Mustela vison, mink
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease sarcoma
Age near term
Comments
Infected with Moloney murine sarcoma virus.
Foci are formed when the cells are superinfected with mink tropic MuLV.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin - 0.03% EDTA solution for 1, remove trypsin and let culture sit at 37C for 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
growth medium, 95%; DMSO, 5%
Name of Depositor PT Peebles
References

Peeples PT, et al. Murine sarcoma virus defectiveness. Viral polymerase expression murine and nonmurine host cells transformed by S+L-type murine sarcoma virus. Virology 67: 344-355, 1975. PubMed: 52940

Basic Documentation
References

Peeples PT, et al. Murine sarcoma virus defectiveness. Viral polymerase expression murine and nonmurine host cells transformed by S+L-type murine sarcoma virus. Virology 67: 344-355, 1975. PubMed: 52940