Human herpesvirus 1 (ATCC® VR-1789)

Classification: Herpesviridae, Simplexvirus  /  Product Format: frozen 1 mL per vial

Classification Herpesviridae, Simplexvirus
Agent Human herpesvirus 1
Strain ATCC-2011-9
Common Name Herpes simplex virus 1
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1789 images
Comments This virus was isolated in cell culture at ATCC.
Effect on Host CPE, refractile cell rounding
Recommended Host Vero (ATCC® CCL-81™)
Growth Conditions Temperature: 37°C

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent.  Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted in VGM to provide an optimal MOI (e.g. MOI 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding VGM.

Incubation: 2-4 days
Effect on Host CPE, refractile cell rounding
Name of Depositor ATCC
Source Clinical patient positive for HSV-1 in Virginia, July 2011