AK-D (ATCC® CCL-150)

Organism: Felis catus, cat  / 

Permits and Restrictions

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Organism Felis catus, cat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age fetus
Complete Growth Medium Ham's F12K medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 3 to 5 minutes, remove trypsin, EDTA leaving enough to wet the cell sheet and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor A Kniazeff
References

. . Lab. Invest. 34: 494-500, 1976.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Lab. Invest. 34: 494-500, 1976.