KG-1a (ATCC® CCL-246.1)

Organism: Homo sapiens, human  /  Cell Type: promyeloblast, Macrophage  /  Tissue: bone marrow  /  Disease: acute myelogenous leukemia

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Organism Homo sapiens, human
Tissue bone marrow
Cell Type promyeloblast, Macrophage
Product Format frozen
Morphology myeloblast
Culture Properties suspension
Biosafety Level 1
Disease acute myelogenous leukemia
Age 59 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The stemline chromosome number is 46 (pseudodiploid), with the 2S component occurring at 5.8%. Seven markers, including five ATCC CCL-246-specific markers, were found in most, if not all metaphases analyzed., Another marker ? del (7) was found only in about 50% of the metaphases. Normal chromosomes 5, 7, 8, 12, 17 and 22 were monosomic. The Y chromosome is detected in the Q-banded preparations.
Derivation
The variant subline KG-1a of the human acute myelogenous leukemia cell line KG-1 was isolated by H.P. Koeffler, et al.
Clinical Data
59 years
Caucasian
male
Comments

After the tenth passage, the parental KG-1 cells were cultured in two separate laboratories within the same department under identical conditions.

After 35 passages the cells in one laboratory expressed morphological differences from the parent line. The variant KG-1a was composed of undifferentiated promyeloblasts.

The cells did not stain for ASD chloroacetate esterase, alpha-naphthyl butyrate esterase or peroxidase.

Both populations exhibit many common characteristics. They share a similar doubling time, are negative for EBNA and VCA, express no surface immunoglobulins and exhibit identical HLA and isoenzyme profiles.

In contrast to the parental KG-1 (ATCC CCL-246) the KG-1a population is unresponsive to colony-stimulating factor in soft-agar culture and does not express the Ia-like antigen.

KG-1a cells are resistant to phorbol diester induced macrophage differentiation and proliferation of the cells is unaffected by the presence of phorbol diesters

.The KG-1a cells are morphologically, cytochemically, and functionally less mature than the parental KG-1.

Although derived from, and almost identical to, KG-1 (ATCC CCL-246) these cells do not spontaneously differentiate to granulocyte and macrophage like cells, do not express DR and do not respond to colony stimulating factor (CSF).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.

Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Twice per week

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Isoenzymes
AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1-2
PGM3, 0
Name of Depositor DW Golde
Passage History
After the tenth passage, the parental KG-1 cells were cultured in two separate laboratories within the same department under identical conditions.
After 35 passages the cells in one laboratory expressed morphological differences from the parent line. The variant KG-1a was composed of undifferentiated promyeloblasts.
References

Koeffler HP. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood 62: 709-721, 1983. PubMed: 6192859

Koeffler HP, et al. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood 56: 265-273, 1980. PubMed: 6967340

Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679

Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Koeffler HP. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood 62: 709-721, 1983. PubMed: 6192859

Koeffler HP, et al. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood 56: 265-273, 1980. PubMed: 6967340

Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679

Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022