Human herpesvirus 2 (ATCC® VR-1779)

Classification: Herpesviridae, Simplexvirus  /  Product Format: frozen 1 mL per vial

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Classification Herpesviridae, Simplexvirus
Agent Human herpesvirus 2
Strain ATCC-2011-2
Common Name Herpes simplex 2
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions  -70°C or colder
Effect on Host
CPE, refractile cell rounding
CPE, refractile cell rounding
Recommended Host

Production Host:  Vero (ATCC® CCL-81)

Vero (ATCC CCL-81)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium, rinse monolayer with plain EMEM. Inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days in a humidified 5% CO2 atmosphere
Effect on Host
CPE, refractile cell rounding
CPE, refractile cell rounding
Name of Depositor ATCC
Source
Clinical sample from a patient positive for HSV-2 in Virginia, July 2011
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation