PFSK-1 (ATCC® CRL-2060)

Organism: Homo sapiens, human  /  Tissue: brain, cerebral hemisphere  /  Disease: malignant primitive neuroectodermal tumor

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Organism Homo sapiens, human
Tissue brain, cerebral hemisphere
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease malignant primitive neuroectodermal tumor
Age 22 months
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor temperature
Karyotype hypotetraploid; 84, XXY; -Y, t(Xp;8q), del(1)(p22), -3, del(4)(p14), -9, -10, -13, -14, -14, -16, -22
Tumorigenic Yes
Effects
Yes, in BALB/c nu/nu mice
Comments

PFSK-1 cells form colonies in soft agar, and lack contact inhibition.

They express the intermediate filament protein, nestin, and are positive for neuron specific enolase (NSE).

They lack characterisics of terminally differentiated neurons or glia.

Restriction fragment length polymorphism studies showed loss of heterozygosity for multiple loci on chromosome 17.

Neither c-myc nor N-myc is amplified or re-arranged.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 times per week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation Complete growth medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 12,14
D16S539: 13
D5S818: 11,12
D7S820: 10
THO1: 9,9.3
TPOX: 8,9
vWA: 17,18
Population Doubling Time 30 hrs
Name of Depositor D Fults
References

Fults D, et al. Establishment and characterization of a human primitive neuroectodermal tumor cell line from the cerebral hemisphere. J. Neuropathol. Exp. Neurol. 51: 272-280, 1992. PubMed: 1316433

Cote GJ, et al. Sequence requirements for regulated RNA splicing of the human fibroblast growth factor receptor-1 alpha exon. J. Biol. Chem. 272: 1054-1060, 1997. PubMed: 8995402

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Fults D, et al. Establishment and characterization of a human primitive neuroectodermal tumor cell line from the cerebral hemisphere. J. Neuropathol. Exp. Neurol. 51: 272-280, 1992. PubMed: 1316433

Cote GJ, et al. Sequence requirements for regulated RNA splicing of the human fibroblast growth factor receptor-1 alpha exon. J. Biol. Chem. 272: 1054-1060, 1997. PubMed: 8995402

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.