Human genomic DNA (HCC2157 BL) (ATCC® CRL-2341D)

Organism: Homo sapiens, human  / 

Permits and Restrictions

View Permits View Restrictions

Product Format frozen Approx.10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Applications
DNA Quality Control Information
High molecular weight DNA is appropriate for polymerase chain reaction (PCR)* process and other molecular biology applications.
Comments
DNA Quality Control Information
>100 kb in size by agarose gel electrophoresis
No RNA detected by agarose gel electrophoresis
A260/A280 ratio >1.8
Tested and verified for PCR amplification and restriction enzyme digestion
High molecular weight DNA is appropriate for polymerase chain reaction (PCR)* process and other molecular biology applications. *The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
High molecular weight DNA isolated from the HCC2157 BL EBV-transformed lymphoblastoid cell line.
Formulation: 1X TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0)
-20C or lower
Biosafety Level 2
Introduction

Cell Line Description:

HCC2157 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV)-transformation of peripheral blood lymphocytes obtained from the same patient as HCC2157.  HCC2157 was initiated from a 48 year old Black female with primary breast ductal carcinoma, and the DNA is available as ATCC CRL-2340D.

Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions The DNA is available with the following restrictions: 1. The CRL-2341 cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor DNA products derived from it may be sold or used for commercial purposes. Nor can the cells nor the DNA be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells and DNA are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the cells, DNA or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.