pSM143 (ATCC® 53038)

Applications: expression vectorshuttle vector  /  Depositors: G Grandi

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Designations pSM143
Depositors G Grandi
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101
Vector Information
Size (kb): 7.3499999046325680
Vector: pSM143 (plasmid)
Promoters: Promoter ErmC
Construction: pUB110, pBR322 ori, fd terminator
Marker(s):ampR,kanR
Construct size (kb): 7.349999904632568
Features: marker(s): ampR
marker(s): kanR
promoter: ErmC
replicon: pMB1, pUB110
restriction site: EcoRI
restriction site: XbaI
terminator: fd
Applications
expression vector
shuttle vector
Comments
An Escherichia coli/Bacillus subtilis shuttle expression vector utilizing the Erm C promoter of pE194 and an fd terminator.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. Japanese Patent 61,257,189 dated Nov 14 1986

Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. European Patent 195303 dated Sep 24 1986

Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. German Federal Republic Patent 3,666,838 dated Dec 14 1989

Guido Grandi, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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