DNA from Chlamydia trachomatis strain UW-36/Cx (ATCC® VR-886D™) Organism: Chlamydia trachomatis Serovar J / General Information Quality Control Specifications Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Product Format frozen >1 µg/mL in 1X TE buffer, 100 µL per vial Storage Conditions Store at -70°C or colder Shipping Information Frozen Applications DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.Sexually Transmitted Disease Research Comments Chlamydia DNA, in total DNA isolated from HeLa-229 cells (ATCC CCL-2.1) infected with Chlamydia trachomatis strain UW-36/Cx (ATCC VR-886). Organism and host cells are also available through the ATCC Catalog. This product was prepared using methods known to inactivate the infecting agent. DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.Organism and host cells are also available through the ATCC Catalog. Focus Area (for hierarchy) Infectious diseases Biosafety Level 1 Quality Control Specifications 1. Store at -20°C or colder. -80°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).2. Concentration is verified as > 1 µg/mL DNA by Picogreen® assay.3. Purity is confirmed by OD260/OD280 ratio (> 1.7) of bulk DNA prior to dilution for product filling.4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis 20 ul of product on a 0.8% agarose gel, visualized by ethidium bromide staining.5. Functional activity is demonstrated by PCR amplification of a 600-12,000 bp amplicon using Chlamydia-specific primers.6. Identity is confirmed by sequencing of ~ 1Kb PCR amplicon. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS