RPMI-7951 ATCC ® HTB-66™ Homo sapiens skin; derived from met

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Tissue	skin; derived from metastatic site: lymph node
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1
Disease	malignant melanoma
Age	18 years
Gender	female
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Karyotype	modal number= 49; range = 47 to 66. This is a hyperdiploid human cell line with the modal chromosome number of 49, occurring in 24% of cells. Polyploid cells occurred at 22%, which is high. Seven marker chromosomes were common to most cells, including t(10p14q); t(15q17p), t(Xp8p), del(17) (p13.1) and three others. Five others occurred only in some cells. Normal X chromosomes were absent. N14, N17 and N22 were mostly single-copied and N2 had three copies. Fluorescence examination did not show the presence of any Y-like chromosome.
Clinical Data	18 years Caucasian female
Antigen Expression	Antigen expression: Blood Type A; Rh+
Genes Expressed	Blood Type A; Rh+
Tumorigenic	Yes
Effects	Yes, in nude mice; forms pigmented melanoma
Comments	A contaminant identified as Mycoplasma fermentans was eliminated in 1975.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Two to three times weekly
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C

STR Profile	Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 11,12 D5S818: 11 D7S820: 11,12 THO1: 9,9.3 TPOX: 8 vWA: 17,19
Isoenzymes	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1

Name of Depositor	G Moore
Year of Origin	1971
References	Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 . . J. Natl. Cancer Inst. 59: 301-307, 1977. Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

Name of Depositor

G Moore

Year of Origin

1971

References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

. . J. Natl. Cancer Inst. 59: 301-307, 1977.

Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cancer Cell Line Mutation Data
References	Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 . . J. Natl. Cancer Inst. 59: 301-307, 1977. Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

RPMI-7951 (ATCC® HTB-66™)

Share this product

Your session will expire in

RPMI-7951 (ATCC^® HTB-66^™)