NCCIT (ATCC® CRL-2073)

Organism: Homo sapiens, human  /  Tissue: embryo, placenta  /  Disease: pluripotent embryonal carcinoma; teratocarcinoma

Organism Homo sapiens, human
Tissue embryo, placenta
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease pluripotent embryonal carcinoma; teratocarcinoma
Age adult
Gender male
Ethnicity Japanese
Images
Derivation
NCCIT was established by Shinichi Teshima (National Cancer Institute, Tokyo, Japan) in 1985 from a mediastinal mixed germ cell tumor.
Clinical Data
adult
Japanese
male
Tumorigenic Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments
This pluripotent stem cell line is capable of somatic and extraembryonic differentiation.
The undifferentiated cells are equivalent to a stage intermediate between seminoma and embryonal carcinoma.
They will differentiate in response to retinoic acid.
NCCIT cells are negative for keratin.
They are positive for vimentin and placental alkaline phosphatase.
The line was contaminated with mycoplasma, but was cured by the depositor prior to deposit at the ATCC.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture two times weekly.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 10,13
D7S820: 10
THO1: 7,9
TPOX: 8
vWA: 14,18
Name of Depositor I Damjanov
Year of Origin 1985
References

Teshima S, et al. Four new human germ cell tumor cell lines. Lab. Invest. 59: 328-336, 1988. PubMed: 2842544

Damjanov I, et al. Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT. Lab. Invest. 68: 220-232, 1993. PubMed: 7680083

Basic Documentation
Other Documentation
References

Teshima S, et al. Four new human germ cell tumor cell lines. Lab. Invest. 59: 328-336, 1988. PubMed: 2842544

Damjanov I, et al. Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT. Lab. Invest. 68: 220-232, 1993. PubMed: 7680083