NULLI-SCC1 (ATCC® CRL-1566)

Organism: Mus musculus, mouse  /  Disease: teratocarcinoma

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Organism Mus musculus, mouse
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease teratocarcinoma
Gender male
Strain 129
Applications
The cells do not differentiate, but can be made to form aggregates and can serve as control for pluripotential teratoma lines (e.
Tested and found negative for ectromelia virus (mousepox).
Clinical Data
male
Comments
The cells do not differentiate, but can be made to form aggregates and can serve as control for pluripotential teratoma lines (e.g. ATCC CRL-1535).
The cells grow best on gelatin coated plates and form flattened colonies.
They will also grow on untreated tissue culture dishes in which case they form rounded colonies and grow more slowly.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution until cells detach. Add fresh medium, aspirate to disperse the cells and centrifuge.
Resuspend the cell pellet in fresh medium and dispense into new gelatin coated flasks at 1 X 10 exp5 cells/sqcm.
Name of Depositor G Martin
References

Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

Martin GR, Evans MJ. Multiple differentiation of clonal teratocarcinoma stem cells following embryoid body formation in vitro. Cell 6: 467-474, 1975.

Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624

Stevens LC. Studies on transplantable testicular teratomas of strain 129 mice. J. Natl. Cancer Inst. 20: 1257-1275, 1958. PubMed: 13549983

. Teratomas and differentiation. New York: Academic Press; 1975.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

Martin GR, Evans MJ. Multiple differentiation of clonal teratocarcinoma stem cells following embryoid body formation in vitro. Cell 6: 467-474, 1975.

Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624

Stevens LC. Studies on transplantable testicular teratomas of strain 129 mice. J. Natl. Cancer Inst. 20: 1257-1275, 1958. PubMed: 13549983

. Teratomas and differentiation. New York: Academic Press; 1975.